Asparagine synthetase regulates the proliferation and differentiation of chicken skeletal muscle satellite cells.

ABSTRACT:

Objective

Asparagine synthetase (ASNS) is an aminotransferase responsible for the biosynthesis of aspartate by using aspartic acid and glutamine. ASNS is highly expressed in fast-growing broilers, but few studies have reported the regulatory role of ASNS in muscle development.

Methods

To explore the function of ASNS in chicken muscle development, the expression of ASNS in different chicken breeds and tissues were first performed by real-time quantitative reverse transcription polymerase chain reaction (RT-PCR). Then, using real-time quantitative RT-PCR, western blot, EdU assay, cell cycle assay and immunofluorescence, the effects of ASNS on the proliferation and differentiation of chicken skeletal muscle satellite cell (SMSC) were investigated. Finally, potential mechanisms

SUBMITTER: Jin H 

PROVIDER: S-EPMC11541025 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature