Comprehensive analysis of lncRNAs modified by m6A methylation in sheep skin.

ABSTRACT:

Objective

N6-methyladenosine (m6A) is the most prevalent methylation of mRNA and plays crucial roles in various physiological processes, including pigmentation. Yet, the regulatory mechanisms, including long noncoding RNAs (lncRNAs) m6A methylation contributing to pigmentation in sheep skin remains unclear. The purpose of this study was to identify potential lncRNAs and the m6A methylation of lncRNAs associated with pigmentation.

Methods

RNA-seq and MeRIP-seq were performed to study the expression of lncRNAs and the m6A methylation of lncRNAs in black and white sheep skin. Furthermore, quantitative real-time polymerase chain reaction (qRT-PCR) was used to verify the consistency with the RNA-seq and MeRIP-seq data.

Results

We identified 168 differentially expressed lnc

SUBMITTER: Meng J 

PROVIDER: S-EPMC11541038 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature