Comparison of primordial germ cell differences at different developmental time points in chickens.

ABSTRACT:

Objective

Recently, the application in the field of germplasm resource conservation has become an important application of primordial germ cells (PGCs). However, due to the lack of deep understanding of the biological characteristics of PGCs at different time points, there is no systematic scheme for the selection of PGCs at which time points in practical application, which affects the practical application effect of PGCs. This study aims to clarify the differences in PGCs during development.

Methods

Here, migration experiment, EdU proliferation assay and cell apoptosis assay were conducted to compare the differences in the migration ability, the proliferation ability and the recovery efficiency among female and male PGCs at E3.5, E4.5, and E5.5, which were explained by the

SUBMITTER: Gong W 

PROVIDER: S-EPMC11541041 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature