Ythdf2 facilitates precursor miR-378/miR-378-5p maturation to support myogenic differentiation.

ABSTRACT: Ythdf2 is known to mediate mRNA degradation in an m⁶A-dependent manner, and it has been shown to play a role in skeletal muscle differentiation. Recently, Ythdf2 was also found to bind to m⁶A-modified precursor miRNAs and regulate their maturation. However, it remains unknown whether this mechanism is related to the regulation of myogenesis by Ythdf2. Here, we observed that Ythdf2 knockdown significantly suppressed myotube formation and impacted miRNAs expression during myogenic differentiation. Through integrated analysis of miRNA and mRNA sequencing data, miR-378 and miR-378-5p were identified as important targets of Ythdf2 in myogenesis. Mechanically, Ythdf2 was found to interact with core components of the pre-miRNA processor complex, namely DICER1 and TARBP2, the

SUBMITTER: Deng K 

PROVIDER: S-EPMC11541164 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature