Comparison of protein immobilization methods with covalent bonding on paper for paper-based enzyme-linked immunosorbent assay.

ABSTRACT: In this study, two methods were examined to optimize the immobilization of antibodies on paper when conducting a paper-based enzyme-linked immunosorbent assay (P-ELISA). Human IgG, as a test-capture protein, was immobilized on paper via the formation of Schiff bases. Aldehyde groups were introduced onto the surface of the paper via two methods: NaIO₄ and 3-aminopropyltriethoxysilane (APTS) with glutaraldehyde (APTS-glutaraldehyde). In the assay, horseradish peroxidase-conjugated anti-human IgG (HRP-anti-IgG) binds to the immobilized human IgG, and the colorimetric reaction of 3,3',5,5'-tetramethylbenzyzine (TMB) produces a blue color in the presence of H₂O₂ and HRP-anti-IgG as a model analyte. The immobilization of human IgG, the enzymatic reaction conditio

SUBMITTER: Chen Y 

PROVIDER: S-EPMC11541255 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature