Triptolide induces hepatotoxicity by promoting ferroptosis through Nrf2 degradation.

ABSTRACT:

Background

Triptolide (TP), a principal active substance from Tripterygium wilfordii, exhibits various pharmacological effects. However, its potential hepatotoxicity has always been a significant concern in clinical applications.

Purpose

This research aimed to explore the involvement of ferroptosis in TP-mediated hepatic injury and the underlying mechanisms.

Methods

In this study, in vitro and in vivo experiments were involved. Hepatocyte damage caused by TP was evaluated using MTT assays, liver enzyme measurement and H&E staining technique. Ferroptosis was assessed by measuring iron level, lipid peroxide, glutathione (GSH), mitochondrial morphology and the key protein/mRNA expression implicated in ferroptosis. To verify the contribution of ferroptosis to TP-induced l

SUBMITTER: Guo L 

PROVIDER: S-EPMC11541276 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature