Dichotomous intronic polyadenylation profiles reveal multifaceted gene functions in the pan-cancer transcriptome.

ABSTRACT: Alternative cleavage and polyadenylation within introns (intronic APA) generate shorter mRNA isoforms; however, their physiological significance remains elusive. In this study, we developed a comprehensive workflow to analyze intronic APA profiles using the mammalian target of rapamycin (mTOR)-regulated transcriptome as a model system. Our investigation revealed two contrasting effects within the transcriptome in response to fluctuations in cellular mTOR activity: an increase in intronic APA for a subset of genes and a decrease for another subset of genes. The application of this workflow to RNA-seq data from The Cancer Genome Atlas demonstrated that this dichotomous intronic APA pattern is a consistent feature in transcriptomes across both normal tissues and various cancer types. Notably,

SUBMITTER: Sun J 

PROVIDER: S-EPMC11541570 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature