Functional characterization of a nanobody-based glycoprotein VI-specific platelet agonist.

ABSTRACT:

Background

Glycoprotein (GP)VI is a platelet-specific collagen receptor required for platelet activation during hemostasis. Platelet reactivity toward collagen is routinely assessed during diagnostic workup of platelet disorders. GPVI can be activated by inducing receptor clustering with suspensions of fibrillar collagen or synthetic cross-linked collagen-related peptide (CRP-XL). However, these suspensions are poorly standardized or difficult to produce. Nanobodies are small recombinant camelid-derived heavy-chain antibody variable regions. They are highly stable, specific, and ideal candidates for developing a stable GPVI agonist for diagnostic assays.

Objectives

Develop a stable nanobody-based GPVI agonist.

Methods

Nanobody D2 (NbD2) was produced as dimers and puri

SUBMITTER: Zivkovic M 

PROVIDER: S-EPMC11541698 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature