Infrared laser-induced gene expression in single cells characterized by quantitative imaging in Physcomitrium patens.

ABSTRACT: A spatiotemporal understanding of gene function requires the precise control of gene expression in each cell. Here, we use an infrared laser-evoked gene operator (IR-LEGO) system to induce gene expression at the single-cell level in the moss Physcomitrium patens by heating a living cell with an IR laser and thereby activating the heat shock response. We identify the laser irradiation conditions that provide higher inducibility with lower invasiveness by changing the laser power and irradiation duration. Furthermore, we quantitatively characterize the induction profile of the heat shock response using a heat-induced fluorescence reporter system after the IR laser irradiation of single cells under different conditions. Our data indicate that IR laser irradiation with long duration leads to h

SUBMITTER: Tomoi T 

PROVIDER: S-EPMC11541703 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature