Singlet oxygen-mediated photochemical cross-linking of an engineered fluorescent flavoprotein iLOV.

ABSTRACT: Genetically encoded photoactive proteins are integral tools in modern biochemical and molecular biological research. Within this tool box, truncated variants of the phototropin two light-oxygen-voltage flavoprotein have been developed to photochemically generate singlet oxygen (¹O₂) in vitro and in vivo, yet the effect of ¹O₂ on these genetically encoded photosensitizers remains underexplored. In this study, we demonstrate that the "improved" light-oxygen-voltage flavoprotein is capable of photochemical ¹O₂ generation. Once generated, ¹O₂ induces protein oligomerization via covalent cross-linking. The molecular targets of protein oligomerization by cross-linking are not endogenous tryptophans or tyrosin

SUBMITTER: Jones BJ 

PROVIDER: S-EPMC11541845 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature