Adipocytes reprogram the proteome of breast cancer cells in organotypic three-dimensional cell cultures.

ABSTRACT: While epidemiological evidence has long linked obesity with an increased risk of breast cancer, the intricate interactions between adipocytes and cancer cells within the tumor microenvironment remain largely uncharted territory. The use of organotypic three-dimensional (3D) cell cultures that more accurately mimic the spatial architecture of tumors represents an innovative approach to this complex issue. In the present study, we investigated the effects of adipocytes on the proteome of Hs578t breast cancer cells cultured in a 3D microenvironment. Using different treatments, we rigorously optimized the experimental conditions to induce the optimal differentiation of 3T3-L1 fibroblasts into mature adipocytes. Then, we grow the Hs578t cells in a simulated microenvironment using an on-top mode

SUBMITTER: Tovar-Hernandez K 

PROVIDER: S-EPMC11542085 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature