PTPN20 promotes metastasis through activating NF-κB signaling in triple-negative breast cancer.

ABSTRACT:

Background

Cancer metastasis remains a major challenge in the clinical management of triple-negative breast cancer (TNBC). The NF-κB signaling pathway has been implicated as a crucial factor in the development of metastases, but the underlying molecular mechanisms remain largely unclear.

Methods

PTPN20 expression was evaluated using data from the Sweden Cancerome Analysis Network-Breast and The Cancer Genome Atlas database, as well as by western blotting and immunohistochemistry in 88 TNBC patients. The ability of PTPN20 to activate NF-κB was assessed by luciferase reporter assays. The effects of PTPN20 overexpression and knockdown via short hairpin RNA were examined in TNBC cell lines by wound healing and transwell matrix penetration assays. Additionally, we analyzed the gr

SUBMITTER: Zuo X 

PROVIDER: S-EPMC11542355 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature