Establishment and application of highly efficient regeneration, genetic transformation and genome editing system for cucurbitacins biosynthesis in Hemsleya chinensis.

ABSTRACT:

Background

Hemsleya Chinensis is a perennial plant in the Cucurbitaceae family containing antibacterial and anti-inflammatory compounds. The lack of genetic transformation systems makes it difficult to verify the functions of genes controlling important traits and conduct molecular breeding in H. chinensis.

Results

Highly efficient calli were induced on MS medium added 1.5 mg·L^- 1 6-benzylaminopurine (6-BA) and 0.02 mg·L^- 1 1-naphthylacetic acid (NAA) with high efficiency (> 95%). The frequency of shoot induction was increased to 90% with a plant growth regulator combination of 1.5 mg·L^- 1 6-BA and 0.1 mg·L^- 1 NAA. Our results also showed that 100% of shoot regeneration was achieved in a shoot regeneration medium. Additionally, mor

SUBMITTER: Wang J 

PROVIDER: S-EPMC11542421 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature