BRWD1 orchestrates small pre-B cell chromatin topology by converting static to dynamic cohesin.

ABSTRACT: Lymphocyte development consists of sequential and mutually exclusive cell states of proliferative selection and antigen receptor gene recombination. Transitions between each state require large, coordinated changes in epigenetic landscapes and transcriptional programs. How this occurs remains unclear. Here we demonstrate that in small pre-B cells, the lineage and stage-specific epigenetic reader bromodomain and WD repeat-containing protein 1 (BRWD1) reorders three-dimensional chromatin topology to affect the transition between proliferative and gene recombination molecular programs. BRWD1 regulated the switch between poised and active enhancers interacting with promoters, and coordinated this switch with Igk locus contraction. BRWD1 did so by converting chromatin-bound static to dynamic co

SUBMITTER: Mandal M 

PROVIDER: S-EPMC11542586 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature