Exploring the Impact of In Vitro-Transcribed mRNA Impurities on Cellular Responses.

ABSTRACT: Advances in mRNA technology have enabled mRNA-based therapies to enter a new era of medicine. Such therapies benefit from a single, standardized in vitro transcription (IVT) manufacturing process applicable to a wide range of targets. This process includes several downstream purification steps, which aim to eliminate impurities that potentially affect safety and efficacy. However, it is not fully understood which impurities are the most critical; hence, some efforts are still needed to establish the correlation between RNA impurities and their role in limiting therapeutic efficacy. To study this relationship, we produced in vitro-transcribed mRNAs using several bacteriophage T7 RNA polymerases, including one wild-type and four engineered variants. Important attributes of the mRNA such as i

SUBMITTER: Camperi J 

PROVIDER: S-EPMC11542617 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature