Monitoring of activity-driven trafficking of endogenous synaptic proteins through proximity labeling.

ABSTRACT: To enable transmission of information in the brain, synaptic vesicles fuse to presynaptic membranes, liberating their content and exposing transiently a myriad of vesicular transmembrane proteins. However, versatile methods for quantifying the synaptic translocation of endogenous proteins during neuronal activity remain unavailable, as the fast dynamics of synaptic vesicle cycling difficult specific isolation of trafficking proteins during such a transient surface exposure. Here, we developed a novel approach using synaptic cleft proximity labeling to capture and quantify activity-driven trafficking of endogenous synaptic proteins at the synapse. We show that accelerating cleft biotinylation times to match the fast dynamics of vesicle exocytosis allows capturing endogenous proteins transie

SUBMITTER: Pascual-Caro C 

PROVIDER: S-EPMC11542813 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature