Metabolic modelling as a powerful tool to identify critical components of Pneumocystis growth medium.

ABSTRACT: Establishing suitable in vitro culture conditions for microorganisms is crucial for dissecting their biology and empowering potential applications. However, a significant number of bacterial and fungal species, including Pneumocystis jirovecii, remain unculturable, hampering research efforts. P. jirovecii is a deadly pathogen of humans that causes life-threatening pneumonia in immunocompromised individuals and transplant patients. Despite the major impact of Pneumocystis on human health, limited progress has been made in dissecting the pathobiology of this fungus. This is largely due to the fact that its experimental dissection has been constrained by the inability to culture the organism in vitro. We present a comprehensive in silico genome-scale metabolic model of Pneumocystis growth and

SUBMITTER: Nev OA 

PROVIDER: S-EPMC11542897 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature