Autophagosome development and chloroplast segmentation occur synchronously for piecemeal degradation of chloroplasts.

ABSTRACT: Plants distribute many nutrients to chloroplasts during leaf development and maturation. When leaves senesce or experience sugar starvation, the autophagy machinery degrades chloroplast proteins to facilitate efficient nutrient reuse. Here, we report on the intracellular dynamics of an autophagy pathway responsible for piecemeal degradation of chloroplast components. Through live-cell monitoring of chloroplast morphology, we observed the formation of chloroplast budding structures in sugar-starved leaves. These buds were then released and incorporated into the vacuolar lumen as an autophagic cargo termed a Rubisco-containing body. The budding structures did not accumulate in mutants of core autophagy machinery, suggesting that autophagosome creation is required for forming chloroplast buds

SUBMITTER: Izumi M 

PROVIDER: S-EPMC11542923 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature