Capillary flow velocity-based length identification of PCR and RPA products on paper microfluidic chips.

ABSTRACT: This work demonstrates a novel, non-fluorescence approach to the length identification of polymerase chain reaction (PCR) and recombinase polymerase amplification (RPA) products, utilizing capillary flow velocities on paper microfluidic chips. It required only a blank paper chip, aminated microspheres, and a smartphone, with a rapid assay time and under ambient lighting. A smartphone evaluated the initial capillary flow velocities on the paper chips for the PCR and RPA products from various bacterial samples, where the pre-loaded aminated microspheres differentiated their flow velocities. Flow velocities were analyzed at different time frames and compared with the instantaneous flow velocities and interfacial tension (γ_LV) data. Subsequent error analysis justified the use of the

SUBMITTER: Buchanan BC 

PROVIDER: S-EPMC11543505 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature