Metabolic profiling unveils enhanced antibacterial synergy of polymyxin B and teixobactin against multi-drug resistant Acinetobacter baumannii.

ABSTRACT: This untargeted metabolomics study investigated the synergistic antibacterial activity of polymyxin B and Leu₁₀-teixobactin, a depsipeptide inhibitor of cell wall biosynthesis. Checkerboard microdilution assays revealed a significant synergy against polymyxin-susceptible and -resistant A. baumannii, excluding lipopolysaccharide-deficient variants. Time-kill assays confirmed bactericidal synergy, reducing bacterial burden by approximately 4-6-log₁₀CFU/mL. The combination (2xMIC polymyxin B and 0.5xMIC Leu₁₀-teixobactin) prevented bacterial regrowth after 24 h, indicating sustained efficacy against the emergence of resistant mutants. The analysis of A. baumannii ATCC™ 19606 metabolome demonstrated that the polymyxin B-Leu₁₀-teixobactin combination

SUBMITTER: Hussein M 

PROVIDER: S-EPMC11543821 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature