Antibacterial and antibiofilm activity of silver nanoparticles stabilized with C-phycocyanin against drug-resistant <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i>.

ABSTRACT:

Background

Biofilms are bacterial communities that can protect them against external factors, including antibiotics. In this study, silver nanoparticles (AgNPs) were formed by modifying AgNPs with C-phycocyanin (Ag-Pc) to inhibit the growth of carbapenem-resistant Pseudomonas aeruginosa (CR P. aeruginosa) and methicillin-resistant Staphylococcus aureus (MRSA) and destroy biofilm of these bacteria.

Methods

The AgNPs were prepared with the green synthesis method, and Pc was used to stabilize the AgNPs. The Ag-Pc's antibacterial and antibiofilm effects were evaluated using the Microbroth dilution method and microtiter plate assay. The inhibitory effect of Ag-Pc on the expression of biofilm-related genes was evaluated by real-time PCR. Moreover, the MTT assa

SUBMITTER: Chegini Z 

PROVIDER: S-EPMC11544008 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature