Photopharmacological modulation of hippocampal local field potential by caged-glutamate with MicroLED probe.

ABSTRACT:

Aim

Photopharmacology is a new technique for modulating biological phenomena through the photoconversion of substances in a specific target region at precise times. Caged compounds are thought to be compatible with photopharmacology as uncaged ligands are released and function in a light irradiation-dependent manner. Here, we investigated whether a microscale light-emitting diode (MicroLED) probe is applicable for the photoconversion of caged-glutamate (caged-Glu) in vivo.

Methods

A needle-shaped MicroLED probe was fabricated and inserted into the mouse hippocampal dentate gyrus (DG) with a cannula for drug injection and a recording electrode for measuring the local field potential (LFP). Artificial cerebrospinal fluid (ACSF) or caged-Glu was infused into the DG and illumina

SUBMITTER: Okada S 

PROVIDER: S-EPMC11544434 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature