Protein profiling of zebrafish embryos unmasks regulatory layers during early embryogenesis.

ABSTRACT: The maternal-to-zygotic transition is crucial in embryonic development, marked by the degradation of maternally provided mRNAs and initiation of zygotic gene expression. However, the changes occurring at the protein level during this transition remain unclear. Here, we conducted protein profiling throughout zebrafish embryogenesis using quantitative mass spectrometry, integrating transcriptomics and translatomics datasets. Our data show that, unlike RNA changes, protein changes are less dynamic. Further, increases in protein levels correlate with mRNA translation, whereas declines in protein levels do not, suggesting active protein degradation processes. Interestingly, proteins from pure zygotic genes are present at fertilization, challenging existing mRNA-based gene classifications. As a

SUBMITTER: da Silva Pescador G 

PROVIDER: S-EPMC11544563 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature