Controlled Translocation of Proteins through a Biological Nanopore for Single-Protein Fingerprint Identification.

ABSTRACT: After the successful sequencing of nucleic acids, nanopore technology has now been applied to proteins. Recently, it has been demonstrated that an electro-osmotic flow can be used to induce the transport of unraveled polypeptides across nanopores. Polypeptide translocation, however, is too fast for accurate reading its amino acid compositions. Here, we show that the introduction of hydrophobic residues into the lumen of the nanopore reduces the protein translocation speed. Importantly, the introduction of a tyrosine at the entry of the nanopore and an isoleucine at the entry of the β-barrel of the nanopore reduced the speed of translocation to ∼10 amino acids/millisecond while keeping a relatively large ionic current, a crucial component for protein identification. These nanopores showed u

SUBMITTER: Sauciuc A 

PROVIDER: S-EPMC11544688 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature