Long-Range Three-Dimensional Tracking of Nanoparticles Using Interferometric Scattering Microscopy.

ABSTRACT: Tracking nanoparticle movement is highly desirable in many scientific areas, and various imaging methods have been employed to achieve this goal. Interferometric scattering (iSCAT) microscopy has been particularly successful in combining very high spatial and temporal resolution for tracking small nanoparticles in all three dimensions. However, previous works have been limited to an axial range of only a few hundred nanometers. Here, we present a robust and efficient measurement and analysis strategy for three-dimensional tracking of nanoparticles at high speed and with nanometer precision. After discussing the principle of our approach using synthetic data, we showcase the performance of the method by tracking gold nanoparticles with diameters ranging from 10 to 80 nm in water, demonstrat

SUBMITTER: Kasaian K 

PROVIDER: S-EPMC11544927 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature