The Impact of Tube Type, Centrifugation Conditions, and Hemolysis on Plasma Circulating MicroRNAs.

ABSTRACT:

Background

In recent years, liquid biopsy has emerged as a promising tool for the diagnosis and prognosis of numerous diseases, including cancer. Among the biomolecules analyzed in liquid biopsies are plasma circulating microRNAs (miRNAs), small non-coding RNAs that have proven to be crucial in the regulation of gene expression and the pathobiology of different health conditions, making them useful as biomarkers. However, variations in preanalytical conditions during biospecimen collection and processing can affect the analytical results.

Objectives

Herein, we determined how the type of collection tube, the number of centrifugations, and the degree of hemolysis can affect plasma circulating miRNA levels.

Methods

A cohort of 11 healthy donors was included. Whole blood

SUBMITTER: Pastor-Navarro B 

PROVIDER: S-EPMC11545111 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature