Unraveling the impact of genome assembly on bacterial typing: a one health perspective.

ABSTRACT:

Background

In the context of pathogen surveillance, it is crucial to ensure interoperability and harmonized data. Several surveillance systems are designed to compare bacteria and identify outbreak clusters based on core genome MultiLocus Sequence Typing (cgMLST). Among the different approaches available to generate bacterial cgMLST, our research used an assembly-based approach (chewBBACA tool).

Methods

Simulations of short-read sequencing were conducted for 5 genomes of 27 pathogens of interest in animal, plant, and human health to evaluate the repeatability and reproducibility of cgMLST. Various quality parameters, such as read quality and depth of sequencing were applied, and several read simulations and genome assemblies were repeated using three tools: SPAdes, Unicycler

SUBMITTER: Merda D 

PROVIDER: S-EPMC11545336 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature