Link Between Individual Codon Frequencies and Protein Expression: Going Beyond Codon Adaptation Index.

ABSTRACT: An important role of a particular synonymous codon composition of a gene in its expression level is well known. There are a number of algorithms optimizing codon usage of recombinant genes to maximize their expression in host cells. Nevertheless, the underlying mechanism remains unsolved and is of significant relevance. In the realm of modern biotechnology, directing protein production to a specific level is crucial for metabolic engineering, genome rewriting and a growing number of other applications. In this study, we propose two new simple statistical and empirical methods for predicting the protein expression level from the nucleotide sequence of the corresponding gene: Codon Expression Index Score (CEIS) and Codon Productivity Score (CPS). Both of these methods are based on the influe

SUBMITTER: Zaytsev K 

PROVIDER: S-EPMC11546221 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature