Matrix Background Screening of an ssDNA Aptamer and Its Identification Against Lactopontin.

ABSTRACT: Lactopontin (LPN) is a highly phosphorylated O-glycosylated acidic protein closely associated with infant gut, brain, and immune development, and its recognition is urgent due to its rising application in fortified dairy products and infant formula. In this study, an ssDNA aptamer against LPN was obtained, among which two kinds of matrix-background-assisted systematic evolution of ligands via exponential enrichment (SELEX) approaches were performed and compared. The direct approach was to utilize the sample matrix as the mixing-incubation background between the ssDNA library and LPN that can theoretically increase screening pressure and simulate practical application scenarios. The indirect approach was to utilize a PBS buffer as a screening background and to include counter-screening step

SUBMITTER: Zhu C 

PROVIDER: S-EPMC11546233 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature