Evaluation of Serum Proteome Sample Preparation Methods to Support Clinical Proteomics Applications.

ABSTRACT: Serum contains several proteins that are associated with disease-related processes. Mass spectrometry (MS)-based proteomics approaches greatly facilitate serum protein biomarker development. However, the serum proteome complexity presents a technical challenge for the accurate, sensitive, and reproducible quantification of proteins by MS. Thus, efficient sample preparation methods are of critical importance for serum proteome analyses. In this study, we evaluated the technical performance of two serum proteome sample preparation methods using sera from patients with high-grade serous ovarian cancer and patients with benign nongynecological conditions with a goal of providing insight into their compatibility with clinical proteomics workflows. One method entailed the use of immobilized tryp

SUBMITTER: Twigg CAI 

PROVIDER: S-EPMC11546599 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature