High-Affinity Plasma Membrane Ca²⁺ Channel Cch1 Modulates Adaptation to Sodium Dodecyl Sulfate-Triggered Rise in Cytosolic Ca²⁺ Concentration in Ogataea parapolymorpha.

ABSTRACT: The cytosolic calcium concentration ([Ca²⁺]_cyt) in yeast cells is maintained at a low level via the action of different transporters sequestrating these cations in the vacuole. Among them, the vacuolar Ca²⁺ ATPase Pmc1 crucially contributes to this process. Its inactivation in Ogataea yeasts was shown to cause sodium dodecyl sulfate (SDS) hypersensitivity that can be alleviated by the inactivation of the plasma membrane high-affinity Ca²⁺ channel Cch1. Here, we show that SDS at low concentrations induces a rapid influx of external Ca²⁺ into cells, while the plasma membrane remains impermeable for propidium iodide. The inactivation of Pmc1 disturbs efficient adaptation to this activity of SDS. The inactivation of Cch1 partially r

SUBMITTER: Kulakova M 

PROVIDER: S-EPMC11546840 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature