New Toolset of Reporters Reveals That Glycogen Granules Are Neutral Substrates of Bulk Autophagy in <i>Komagataella phaffii</i>.

ABSTRACT: Glycogen, a branched polysaccharide organized into glycogen granules (GGs), is delivered from the cytoplasm to the lysosomes of hepatocytes by STBD1-driven selective autophagy (glycophagy). Recently, we developed Komagataella phaffii yeast as a simple model of GG autophagy and found that it proceeds non-selectively under nitrogen starvation conditions. However, another group, using Saccharomyces cerevisiae as a model, found that glycogen is a non-preferred cargo of nitrogen starvation-induced bulk autophagy. To clarify cargo characteristics of K. phaffii GGs, we used the same glycogen synthase-based reporter (Gsy1-GFP) of GG autophagy in K. phaffii as was used in S. cerevisiae. The K. phaffii Gsy1-GFP marked the GGs and reported on their autophagic

SUBMITTER: Wijewantha NV 

PROVIDER: S-EPMC11546884 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature