Study on the Function of Leptin Nutrient Acquisition and Energy Metabolism of Zebrafish (<i>Danio rerio</i>).

ABSTRACT: Leptin plays an indispensable role in energy homeostasis, and its involvement in metabolic activities has been extensively explored in fish. We generated mutant lines of leptina (-5 bp) and leptinb (+8 bp) in zebrafish using CRISPR/Cas9 technology to explore the metabolic characteristics of lepa and lepb mutant zebrafish in response to high glucose nutritional stress induced by high levels of carbohydrates. The results were as follows: the body weight and food intake of adult zebrafish of the two mutant species were increased; the visceral fat accumulation, whole-body crude lipid, and crude protein contents of lepb^-/- were increased; and the visceral fat accumulation and crude lipid in lepa^-/- zebrafish were decreased. The blo

SUBMITTER: Wu J 

PROVIDER: S-EPMC11546987 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature