High-Level Production of a Recombinant Protein in <i>Nicotiana benthamiana</i> Leaves Through Transient Expression Using a Double Terminator.

ABSTRACT: Various bio-based recombinant proteins have been produced for industrial, medical, and research purposes. Plants are potential platforms for recombinant protein production because of several advantages. Therefore, establishing a system with high target gene expression to compensate for the low protein yield of plant systems is crucial. In particular, selecting and combining strong terminators is essential because the expression of target genes can be substantially enhanced. Here, we aimed to quantify the enhancement in the fluorescence intensity of the turbo green fluorescence protein (tGFP) caused by the best double-terminator combinations compared to that of the control vector using agroinfiltration in Nicotiana benthamiana leaves. tGFP fluorescence increased by 4.1-fold in leaf s

SUBMITTER: Lee J 

PROVIDER: S-EPMC11547012 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature