Single-Cell Multiomics Profiling Reveals Heterogeneity of Muller Cells in the Oxygen-Induced Retinopathy Model.

ABSTRACT:

Purpose

Retinal neovascularization poses heightened risks of vision loss and blindness. Despite its clinical significance, the molecular mechanisms underlying the pathogenesis of retinal neovascularization remain elusive. This study utilized single-cell multiomics profiling in an oxygen-induced retinopathy (OIR) model to comprehensively investigate the intricate molecular landscape of retinal neovascularization.

Methods

Mice were exposed to hyperoxia to induce the OIR model, and retinas were isolated for nucleus isolation. The cellular landscape of the single-nucleus suspensions was extensively characterized through single-cell multiomics sequencing. Single-cell data were integrated with genome-wide association study (GWAS) data to identify correlations between ocular cell t

SUBMITTER: Yao X 

PROVIDER: S-EPMC11547256 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature