Interaction Dynamics of Plant-Specific Insert Domains from Cynara cardunculus: A Study of Homo- and Heterodimer Formation.

ABSTRACT: Plant aspartic proteinases (APs) from Cynara cardunculus feature unique plant-specific insert (PSI) domains, which serve as essential vacuolar sorting determinants, mediating the transport of proteins to the vacuole. Although their role in vacuolar trafficking is well established, the exact molecular mechanisms that regulate PSI interactions and functions remain largely unknown. This study explores the ability of PSI A and PSI B to form homo- and heterodimers using a combination of pull-down assays, the mating-based split-ubiquitin system (mbSUS), and FRET-FLIM analyses. Pull-down assays provided preliminary evidence of potential PSI homo- and heterodimer formation. This was conclusively validated by the more robust in vivo mbSUS and FRET-FLIM assays, which clearly demonstrated the

SUBMITTER: Sampaio M 

PROVIDER: S-EPMC11547502 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature