ZIP8 Is Upregulated in the Testis of Zip14^-/- Mice.

ABSTRACT:

Background/objectives

Manganese is an essential nutrient involved in various biological processes, including reproductive health, yet the mechanisms regulating its homeostasis in the testis remain poorly understood.

Methods and results

In this study, we investigated the expression and regulation of key manganese transporters-ZIP8, ZIP14, and ZnT10-in mouse testes. Immunoblotting analyses revealed that ZIP8 is expressed in the testes, while ZIP14 and ZnT10 were undetectable. Using Zip14 knockout (Zip14^-/-) mice, which exhibit systemic manganese overload, we discovered a significant increase in manganese levels in the testis, accompanied by an upregulation of ZIP8. Importantly, the levels of other essential metals, such as iron, zinc, and copper, remai

SUBMITTER: Vungutur V 

PROVIDER: S-EPMC11547875 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature