Efficacy of Plant Tissue Culture Techniques for Eliminating Black Mulberry Idaeovirus (BMIV) from Infected Black Mulberry (<i>Morus nigra</i>).

ABSTRACT: Obtaining virus-free plants is a crucial step in disease management that enables reliable and profitable fruit farming. The present study applied various in vitro virus elimination protocols, including apical shoot culture, chemotherapy, thermotherapy, cryotherapy, and their combination, to eliminate black mulberry Idaeovirus (BMIV) from sour black mulberry. First, a shoot tip (0.5-2 mm) culture protocol was optimized, and four ribavirin concentrations (0, 10, 20, and 30 mg/L) were investigated over five weeks as a form of chemotherapy (ch). For the first thermotherapy treatment (Ch + Th^1st), chemotherapy treatment was followed by a gradual increase in the temperature (24-33 °C). In another experiment (Th^2nd + Ch), in vitro shoots were incubated in the dark for two we

SUBMITTER: Abdelwahab Elansary DW 

PROVIDER: S-EPMC11548174 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature