Adaptation of a Model Spike Aptamer for Isothermal Amplification-Based Sensing.

ABSTRACT: Isothermal amplification (IA) techniques like rolling circle amplification (RCA) and loop-mediated isothermal amplification (LAMP) have gained significant attention in recent years due to their ability to rapidly amplify DNA or RNA targets at a constant temperature without the need for complex thermal cycling equipment. Such technologies, combined with colorimetric systems rendering visual confirmation of the amplification event, are ideal for the development of point-of-need detection methods suitable for field settings where access to specialized laboratory equipment is limited. The utility of these technologies, thus far limited to DNA and RNA targets, could be broadened to a wide range of targets by using aptamers. Composed of DNA or RNA themselves, aptamers can bind to substances, inc

SUBMITTER: Yurdusev E 

PROVIDER: S-EPMC11548252 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature