Coassembly of a Hybrid Synthetic-Biological Chitosan-<i>g</i>-Poly(<i>N</i>-isopropylacrylamide) Copolymer with DNAs of Different Lengths.

ABSTRACT: Natural polysaccharides can serve as carriers of genes owing to their intrinsic biocompatibility, biodegradability, and low toxicity. Additionally, they can be easily chemically modified, e.g., through grafting, leading to hybrid synthetic-biological copolymers with additional functionalities. In this work we report on the electrostatic interaction between a chitosan-g-poly(N-isopropylacrylamide) (Chit-g-PNIPAM) copolymer and DNA macromolecules of different lengths (i.e., 50 and 2000 bp), towards the construction of polyplexes that can serve as potential gene delivery systems. At the basic science level, the work aims to elucidate the effects of DNA length on the structural and physicochemical properties of the thermoresponsive hybrid macromolecular assemblies. The pro

SUBMITTER: Karayianni M 

PROVIDER: S-EPMC11548708 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature