In vitro one-pot construction of influenza viral genomes for virus particle synthesis based on reverse genetics system.

ABSTRACT: The reverse genetics system, which allows the generation of influenza viruses from plasmids encoding viral genome, is a powerful tool for basic research on viral infection mechanisms and application research such as vaccine development. However, conventional plasmid construction using Escherichia coli (E.coli) cloning is time-consuming and has difficulties handling DNA encoding genes toxic for E.coli or highly repeated sequences. These limitations hamper rapid virus synthesis. In this study, we establish a very rapid in vitro one-pot plasmid construction (IVOC) based virus synthesis. This method dramatically reduced the time for genome plasmid construction, which was used for virus synthesis, from several days or more to about 8 hours. Moreover, infectious viruses could be synthesized with

SUBMITTER: Tanaka R 

PROVIDER: S-EPMC11548778 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature