In silico and functional analysis identifies key gene networks and novel gene candidates in obesity-linked human visceral fat.

ABSTRACT:

Objective

Visceral adiposity is associated with increased proinflammatory activity, insulin resistance, diabetes risk, and mortality rate. Numerous individual genes have been associated with obesity, but studies investigating gene regulatory networks in human visceral obesity have been lacking.

Methods

We analyzed gene regulatory networks in human visceral adipose tissue (VAT) from 48 and 11 Chinese patients with and without obesity, respectively, using gene coexpression and gene regulatory network construction from RNA-sequencing data. We also conducted RNA interference-based functional tests on selected genes for effects on adipocyte differentiation.

Results

A scale-free gene coexpression network was constructed from 360 differentially expressed genes between VAT sa

SUBMITTER: Wang L 

PROVIDER: S-EPMC11548800 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature