Ergotamine Targets KIF5A to Facilitate Anoikis in Lung Adenocarcinoma.

ABSTRACT:

Background

Kinesin family member 5A (KIF5A) has been reported to be closely related to cancer progression. The aim of this study was to investigate the effect of KIF5A on lung adenocarcinoma (LUAD) and its potential molecular mechanisms.

Methods

Using bioinformatics analysis methods and molecular experiments, the expression of KIF5A in LUAD was analyzed, with its expression in attached and detached tumor cells assessed. Gene set enrichment analysis (GSEA) of KIF5A was carried out. The small molecular drug with the highest affinity for KIF5A was screened out through molecular docking experiments, which was validated through cellular thermal shift assay (CETSA). Quantitative polymerase chain reaction (qPCR) was employed to measure the expression levels of anoikis-repressing ge

SUBMITTER: Bao B 

PROVIDER: S-EPMC11549061 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature