SCAR-6 elncRNA locus epigenetically regulates PROZ and modulates coagulation and vascular function.

ABSTRACT: In this study, we characterize a novel lncRNA-producing gene locus that we name Syntenic Cardiovascular Conserved Region-Associated lncRNA-6 (scar-6) and functionally validate its role in coagulation and cardiovascular function. A 12-bp deletion of the scar-6 locus in zebrafish (scar-6^{gib007Δ12/Δ12}) results in cranial hemorrhage and vascular permeability. Overexpression, knockdown and rescue with the scar-6 lncRNA modulates hemostasis in zebrafish. Molecular investigation reveals that the scar-6 lncRNA acts as an enhancer lncRNA (elncRNA), and controls the expression of prozb, an inhibitor of factor Xa, through an enhancer element in the scar-6 locus. The scar-6 locus suppresses loop formation between prozb and scar-6 sequences, which might be facilitated by the methylation of C

SUBMITTER: Ranjan G 

PROVIDER: S-EPMC11549340 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature