Identification strategy of wild and cultivated Astragali Radix based on REIMS combined with two-dimensional LC-MS.

ABSTRACT: A rapid and real-time method was established based on the combination of rapid evaporative ionization mass spectrometry (REIMS) and two-dimensional liquid chromatography mass spectrometry (2DLC-MS) for identification of wild Astragali Radix (WAR) and cultivated AR (CAR). The samples were analyzed under ambient ionization without time-consuming sample preparation. The phenotypic data of WAR and CAR were used to develop a real-time recognition model. Subsequently, the compounds in these two species were comprehensively characterized based on 2DLC-MS, and 45 different compounds were screened out by multivariate statistical analysis. A semi-quantitative method for 45 different compounds was established based on ultrahigh-performance liquid chromatography/quadrupole-linear ion trap mass spectro

SUBMITTER: Chen S 

PROVIDER: S-EPMC11549423 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature