De novo biosynthesis and nicotinamide biotransformation of nicotinamide mononucleotide by engineered yeast cells.

ABSTRACT: β-Nicotinamide mononucleotide (NMN) is a precursor of NAD⁺ in mammals. Research on NAD⁺ has demonstrated its crucial role against aging and disease. Here two technical paths were established for the efficient synthesis of NMN in the yeast Pichia pastoris, enabling the production of NMN from the low-cost nicotinamide (NAM) or basic carbon sources. The yeast host was systematically modified to adapt to the biosynthesis and accumulation of NMN. To improve the semi-biosynthesis of NMN from NAM, nicotinamide phosphoribosyltransferases were expressed intracellular to evaluate their catalytic activities. The accumulation of extracellular NMN was further increased by the co-expression of an NMN transporter. Fine-tuning of gene expression level produced 72.1 mg/L NMN from NAM

SUBMITTER: Ren Y 

PROVIDER: S-EPMC11549688 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature