Growth Hormone Neuroprotective Effects After an Optic Nerve Crush in the Male Rat.

ABSTRACT:

Purpose

Growth hormone (GH) has neuroprotective effects that have not been evaluated in the mammalian visual system. This study tested the hypothesis that GH administration can promote retinal neuroprotection in an optic nerve crush (ONC) model in male rats.

Methods

The ON was compressed for 10 seconds, and bovine GH was injected concomitantly to injury for 14 days (0.5 µg/g every 12 hours). At 24 hours and 14 days after ONC, we evaluated the effects of GH upon several markers by quantitative PCR (qPCR), Western blot, and immunohistochemistry; the ON integrity was assessed using CTB Alexa 488 anterograde tracer, and retinal function was tested by full-field electroretinogram.

Results

GH partially prevented the ONC-induced death of retinal ganglion cells (RGCs), as wel

SUBMITTER: Epardo D 

PROVIDER: S-EPMC11549927 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature