Structure-guided design and evaluation of CRM197-scaffolded vaccine targeting GnRH for animal immunocastration.

ABSTRACT: Immunocastration is a humane alternative to surgical castration for controlling population and estrous behaviors in animals. Gonadotropin-releasing hormone (GnRH), the pivotal initiating hormone of the hormonal cascade in mammals, is the optimal target for immunocastration vaccine development. Cognate antigen-mediated cross-linking of B cell receptors (BCRs) is a strong activation signal for B cells and is facilitated by repetitive surface organizations of antigens. In this study, we describe the structure-guided design of highly immunogenic chimeric proteins with variant numbers of GnRH peptide insertion by epitope grafting. Linear B-cell epitopes of cross-reacting material 197 (CRM197) were replaced with multiple copies of GnRH peptide, and the inserts were displayed on the surface of th

SUBMITTER: Duan Y 

PROVIDER: S-EPMC11550287 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature