Multiplexed expansion revealing for imaging multiprotein nanostructures in healthy and diseased brain.

ABSTRACT: Proteins work together in nanostructures in many physiological contexts and disease states. We recently developed expansion revealing (ExR), which expands proteins away from each other, in order to support better labeling with antibody tags and nanoscale imaging on conventional microscopes. Here, we report multiplexed expansion revealing (multiExR), which enables high-fidelity antibody visualization of >20 proteins in the same specimen, over serial rounds of staining and imaging. Across all datasets examined, multiExR exhibits a median round-to-round registration error of 39 nm, with a median registration error of 25 nm when the most stringent form of the protocol is used. We precisely map 23 proteins in the brain of 5xFAD Alzheimer's model mice, and find reductions in synaptic protein clu

SUBMITTER: Kang J 

PROVIDER: S-EPMC11550395 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature