MIMt: a curated 16S rRNA reference database with less redundancy and higher accuracy at species-level identification.

ABSTRACT:

Motivation

Accurate determination and quantification of the taxonomic composition of microbial communities, especially at the species level, is one of the major issues in metagenomics. This is primarily due to the limitations of commonly used 16S rRNA reference databases, which either contain a lot of redundancy or a high percentage of sequences with missing taxonomic information. This may lead to erroneous identifications and, thus, to inaccurate conclusions regarding the ecological role and importance of those microorganisms in the ecosystem.

Results

The current study presents MIMt, a new 16S rRNA database for archaea and bacteria's identification, encompassing 47 001 sequences, all precisely identified at species level. In addition, a MIMt2.0 version was created with only

SUBMITTER: Cabezas MP 

PROVIDER: S-EPMC11550520 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature